Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi † †Electronic supplementary information (ESI) available: Figures and tables. See DOI: 10.1039/c5sc03340c Click here for additional data file.
نویسندگان
چکیده
Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the model organism Vibrio harveyi by bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition of V. harveyi from individual- to group-behaviors. We identified 176 quorum-sensing-regulated proteins at early, intermediate, and late stages of the transition, and we mapped the temporal changes in quorum-sensing proteins controlled by both transcriptional and post-transcriptional mechanisms. Analysis of the identified proteins revealed 86 known and 90 new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis.
منابع مشابه
Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi
Division of Chemistry and Chemical Engine Pasadena, CA 91125, USA. E-mail: tirrell@c Department of Molecular and Cellul Bloomington, IN 47405, USA Proteome Exploration Laboratory, Beck Technology, Pasadena, CA 91125, USA Center for Genome Sciences and Systems B Medicine, St. Louis, MO 63108, USA Department of Molecular Biology, Princeton Howard Hughes Medical Institute, Chevy C † Electronic sup...
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